BBa_K187004

BBa_K187004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K187004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team BioBytes
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:07

Sigma 70 10% promoter in pAB, Biobytes plasmid



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K187004_sequence (Version 1)

Description

The 10% promoter produces ~10% of the expression level from a consensus promoter. The pAB plasmid (part BBa_K187000) adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes BBF RFC for details on this method.

Notes

The 1% promoter produces ~1% of the expression level from a consensus promoter. The pAB plasmid adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes RFC for details on this method.

The Biobytes assembly method requires that the DNA fragments to be assembled have long sticky ends of particular sequence. DNA fragments with these sticky ends can be prepared using genes in pBA or pAB as a template. The structure of pAB and pBA will result in a ribosome binding site being included 7bp upstream of the start codon of the gene. It is not necessary to include a promoter with the gene when cloning it into pAB or pBA. Instead, a promoter with long sticky ends can be prepared from separate pAB and pBA plasmids just containing promoters. The desired promoter can then be assembled upstream of a gene using the BioBytes method. This abilty to easily 'mix and match' promoters and genes allows gene expresison to be standardized and optimized.

Unlikely standard cloning that would requires several days to insert a promoter into a plasmid upstream of a gene, the addition of one DNA segment with the BioBytes method takes only 20minutes.

Source

10% promoter was modified from Anderson collection of promoters, part BBa_J23114. Plasmid pAB was derived from pUC19, and is entered as part BBa_K187000.

Sequence Annotation Location Component / Role(s)
XbaI
-35
-10
PstI
10,15
16,21
47,52
40,45
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K187004/1