Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team BioBytes
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:07
Sigma 70 100% promoter in pAB, BioBytes plasmid
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K187008_sequence (Version 1) |
Description
The 100% promoter has a sigma 70 consensus sequence, and should produce maximal expression. The pAB plasmid (part BBa_K187000) adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes BBF RFC for details on this method.Notes
The 100% promoter has a sigma 70 consensus sequence, and should produce maximal expression. The pAB plasmid adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes RFC for details on this method.The Biobytes assembly method requires that the DNA fragments to be assembled have long sticky ends of particular sequence. DNA fragments with these sticky ends can be prepared using genes in pBA or pAB as a template. The structure of pAB and pBA will result in a ribosome binding site being included 7bp upstream of the start codon of the gene. It is not necessary to include a promoter with the gene when cloning it into pAB or pBA. Instead, a promoter with long sticky ends can be prepared from separate pAB and pBA plasmids just containing promoters. The desired promoter can then be assembled upstream of a gene using the BioBytes method. This abilty to easily 'mix and match' promoters and genes allows gene expresison to be standardized and optimized.
Unlike standard cloning that would requires several days to insert a promoter into a plasmid upstream of a gene, the addition of one DNA segment with the BioBytes method takes only 20minutes.