BBa_K1885987

BBa_K1885987 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1885987
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kien Patrick Malarney
Date created: 2016-10-28 11:00:00
Date modified: 2016-10-29 11:24:24

Novel osmY-PETase fusion protein, A0.58



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1885987_sequence (Version 1)

Description

PETase is a novel enzyme for the degradation of polyethylene terephthalate (PET) plastic. It was discovered in Ideonella sakaiensis, a bacteria species found by screening PET-exposed microbe communities in Japan. It functions as the first step in the degradation of PET; PETase catalyzes the hydrolysis of PET into terephthalate and ethylene glycol monomers. The N-terminal osmY protein and its signal peptide fused to PETase, with serine-glycine peptide repeats intervening, serves to secrete the PETase out of the cell so that it can act on PET in the cell's local environment. The Anderson promoter with a relative strength of 0.58 that precedes the fusion protein serves to constitutively express the fusion PETase, enhancing the degradation of environmental PET by the presence of more fusion PETase by constitutive expression. This part offers a more efficient initial step for teams interested in the bioremediation of PET, because the first step involved in PET bioremediation is hydrolysis into products that can then be utilized by a cell. Teams can then metabolically engineer bacteria of interest to metabolize ethylene glycol and terephthalate, but initially, these monomers must be produced by hydrolysis. Thus, PET bioremediation depends on the production of metabolizable monomers, and this part offers more efficient production of such monomers.

Notes

We added a serine-glycine linker sequence between the osmY and PETase sequences to minimize the chances of the domains interfering with one another???s folding.

Source

The osmY and PETase coding sequences were originally drawn from the escherichia coli and ideonella sakainesis genome sequences, respectively.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1885987/1