Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pei-Chuan Chu
Date created: 2009-09-23 11:00:00
Date modified: 2015-05-08 01:11:13
no.1
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K188774_sequence (Version 1) |
Description
To inhibit transcription of the LacI promoter (pLacl, BBa_R0010), we have to encode LacI protein (BBa_C0012). LacI protein can bind to pLacI and repress the expression, but lactose, which repress lacI binding, act as a switch opening the expression. Therefore, the inter-regulation could be a timer device, so LacI is a key component of first device. The second gene is the LuxR, which is a membrane receptor, takes external AHL compound to pass down the signals. We use the LuxR as our bacteria-sensor to sense other bacteria in the environment. For selecting other parts, we choose one kind of constitutive promoter family(BBa_J23106) as the promoter, and the expression amount of this promoter is moderate comparing others in the family. Furthermore, we use the commom ribosome-binding site(BBa_B0034) and the terminator(BBa_B0014), which is a special double terminator, to ensure the transcription would exactly stop.Notes
zxcSource
zxc| Access | Instance | Definition |
| public public public public public public | BBa_J23106 BBa_B0034 BBa_C0012 BBa_B0034 BBa_C0062 BBa_J61048 | BBa_J23106 BBa_B0034 lacI BBa_B0034 luxr BBa_J61048 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_B0034 BBa_C0012 BBa_B0034 BBa_C0062 BBa_J61048 | 1,35 44,55 62,1189 1223,1234 1241,1996 2030,2142 | BBa_J23106 BBa_B0034 lacI BBa_B0034 luxr BBa_J61048 |