BBa_K1889007

BBa_K1889007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1889007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lee Chi Hong
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 07:38:27

Express amino acid deaminase under constitutive promoter



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1889007_sequence (Version 1)

Description

K1889007 is a gene coding for an amino acid deaminase gene. It is derived from Proteus mirabilis. It functions to turn phenylalanine into phenylpyruvic acid by removing its amino group, coming from the enzyme family that catalyzes the breakdown of amino acids into alpha-keto acids. In our experiment, this gene is used to turn phenylalanine, a naturally occurring amino acid in E. coli, into phenylpyruvic acid. The phenylpyruvic acid then acts as an attractant to C. elegans.

Notes

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Source

The sequence of the gene was referenced from an article from Journal of Bacteriology, titled ???Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad.??? The DNA sequence was slightly modified to allow its chemical synthesis by IDT while still retaining the original amino acid sequence.

Massad, G., Zhao, H., & Mobley, H. L. (1995). Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad. Journal of Bacteriology, 177(20), 5878???5883.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1889007/1