Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 01:36:56
alpha-tubulin
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1891000_sequence (Version 1) |
Description
It encodes alpha-tubulin from human breast cancer cell line Mcf-7. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of alpha-tubulinNotes
To get this part, we extracted human breast cancer cell line Mcf-7 cDNA. And then we designed primers to get this gene fragment with prefix and suffix. Because the sequence of alpha -tubulin have tow EcoRI restriction enzyme cutting sites. So we use point mutation to mutate these sites.Source
We get this gene from human breast cancer cell line Mcf-7 cDNA using PCR.| Sequence Annotation | Location | Component / Role(s) |
| alpha-tubulin | 1,1356 | feature/misc sequence_feature |