BBa_K1891003

BBa_K1891003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1891003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 04:12:34

N-Luciferase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1891003_sequence (Version 1)

Description

It encodes N-terminal of luciferase. When it meets C-terminal of luciferase, the N-terminal and C-terminal can become a complete luciferase. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of N-Luciferase. We use it as a part of reporter gene to detect whether it has taxol or its analogues.

Notes

To get this part, we designed primers according to the gene sequence and then cloned it to our expression vector.

Source

We get this gene from pCambia1300-N-Luciferase plasmid.

Sequence Annotation Location Component / Role(s)
N-Luciferase
1,1248
sequence_feature feature/misc
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1891003/1