Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 09:27:47
C-Luciferase+alpha-tubulin
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1891006_sequence (Version 1) |
Description
It expresses C-Luciferase-alpha-tubulin fusion protein. The C-terminal of C-Luciferase links to the N-terminal of alpha-tubulin. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein.Notes
We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and C-Luciferase gene fragment. And then we redesigned a antisense primer of C-Luciferase and a sense primer of alpha-tubulin with a linker about 42bp. And then doing fusion PCR.Source
C-Luciferase comes from pCambia1300-C-Luciferase plasmid. Alpha-tubulin comes from human breast cancer cell line Mcf-7 cDNA.| Sequence Annotation | Location | Component / Role(s) |
| C-luciferase linker alpha-tubulin | 1,459 459,501 502,1857 | feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc |