BBa_K1894001

BBa_K1894001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1894001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Qinyu Ge

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-19 01:30:05

shuttle plasmid which can replicate in cyanobacteria and E.coli

Details

• Types: DnaRegion
• Roles: Project; engineered_region
• Sequences: BBa_K1894001_sequence (Version 1)

Description

The shuttle plasmid can both replicate in cyanobacteria and E.coli.
The shuttle plasmid pPKE2 we used possesses the replication origins of E.coli plasmid and cyanobacterium plasmid, CaMV35S promoter, multiple cloning sites (MCS) and rbcS polyA terminator, Which makes it convenient to insert and express target gene and to screen out the recombinants. Ori site of cyanobacterium plasmid comes from indigenous plasmids pPbs extracted from Plectonema boryanum.

Notes

We introduce shuttle plasmid into our experiments because we need to transform both E.coli and microcystis aeruginosa during experiments. （The plasmid of microcystis aeruginosa itself does not contain selectable marker and cannot replicate in E.coli. Traditional plasmid vector used to transform E.coli cannot transform microcystis either.

Source

Ori site of cyanobacterium plasmid comes from indigenous plasmids pPbs extracted from Plectonema boryanum.

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1894001/1

Member of these Collections

• iGEM Parts Registry