mSA2

BBa_K1896000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1896000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bob Van Hove, Maarten Van Brempt

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-18 05:42:45

Monomeric Streptavidin (mSA2)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K1896000_sequence (Version 1)

Description

Monomeric streptavidin, to be used in protein fusions.

Lim, K. H., Huang, H., Pralle, A., & Park, S. (2013). Stable, high‐affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnology and bioengineering, 110(1), 57-67.

Notes

todo

Source

Combination of streptavidin (Streptomyces avidinii) and rhizavidin (Rhizobium etli), engineered through homology modeling (doi:10.1002/bit.24605).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mSA2 coding region	22,380	CDS, feature/cds
GSTGS linker	7,21	feature/cds, CDS
start	1,3	start_codon, feature/start
NgoMIV	4,9	sequence_feature, feature/dna
AgeI	379,384	feature/dna, sequence_feature
stop	385,387	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1896000/1

Member of these Collections

• iGEM Parts Registry