BBa_K1897011

BBa_K1897011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1897011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ang Shi Hui

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-10 12:54:39

Invasin

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1897011_sequence (Version 1)

Description

This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, Invasin coding sequence BBa_K1897010 and double terminator BBa_B0015.

Notes

There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS BBa_K1897010 is shifted to after the HA tag.

Source

Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [https://www.ncbi.nlm.nih.gov/nuccore/M17448.1 [Genbank M17448.1]] (see BBa K1897010. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062	13,67	engineered_region, feature/BioBrick
BBa_B0030	68,82	engineered_region, feature/BioBrick
BBa_K1897010	91,3048	engineered_region, feature/BioBrick
HA tag	3049,3075	sequence_feature, feature/misc
HA tag	3076,3102	feature/misc, sequence_feature
Stop Codon	3103,3105	feature/stop, stop_codon
BBa_B0015	3106,3234	engineered_region, feature/BioBrick

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1897011/1

Member of these Collections

• iGEM Parts Registry