Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Roger Milton Rubio S??nchez, Enrique Chimal, Enrique Palomino, Brian Morteo
Date created: 2016-06-06 11:00:00
Date modified: 2016-06-07 09:08:40
TAT signal peptide with inducible promoter and RBS
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K1904008_sequence (Version 1) |
Description
IPTG inducible promoter and RBS (BBa_J04500) was assemble with BBa_K1904007, resulting on the TAT signal peptide for protein export in Outer Membrane Vesicles (OMVs). This biobrick is reported in RFC25 to allow protein engineering avoiding stop codons in between the protein of interest and the peptide.Notes
This biobrick was designed and optimised for its usage in E. coli and in order to have it chemically synthesised.Source
E. coli| Sequence Annotation | Location | Component / Role(s) |
| scar BBa_R0010 CAP -35 -10 LacI start BBa_B0034 BBa_K1904007 scar | 221,226 1,200 89,126 137,142 161,166 166,200 173,173 209,220 227,316 201,208 | feature/scar feature/BioBrick engineered_region non_covalent_binding_site feature/binding promoter feature/promoter promoter feature/promoter feature/binding non_covalent_binding_site start_codon feature/start feature/BioBrick engineered_region engineered_region feature/BioBrick feature/scar |