Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lisa R??ttjers
Date created: 2016-09-21 11:00:00
Date modified: 2016-09-22 06:18:00
chiA for Varroa destructor
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1913000_sequence (Version 1) |
Description
Serratia marcescens GEI chiA protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiA has both exochitinase and endochitinase activity. This protein is known to show synergistic activity with ChiB. ChiA can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiA.Notes
This sequence was optimized using the IDT codon optimization tool. Multiple restriction sites were removed; BsaI at position 195, PstI at position 644, PstI at position 905 and EcoRI at position 943. Additionally, several antisense stop codons were introduced to prevent antisense transcription.Source
The source of this part is chiA from Serratia marcescens GEI; accession GQ855217.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon TAA stop codon TAA stop codon chiA coding region | 1,3 1690,1692 1693,1695 1,1695 | start_codon feature/start stop_codon feature/stop feature/stop stop_codon CDS feature/cds |