Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lisa R??ttjers
Date created: 2016-09-21 11:00:00
Date modified: 2016-09-22 06:27:24
chiB for Varroa destructor
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1913001_sequence (Version 1) |
Description
Serratia marcescens GEI chiB protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiB has exochitinase activity. This protein is known to show synergistic activity with ChiA. ChiB can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiB.Notes
This sequence was optimized using the IDT codon optimization tool. One restriction site was removed; PstI at position 1049. Additionally, several antisense stop codons were introduced to prevent antisense transcription.Source
This part is based on chiB from Serratia marcescens GEI; accession GQ855218.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon TAA stop codon TAA stop codon chiB coding region | 1,3 1498,1500 1501,1503 1,1503 | feature/start start_codon feature/stop stop_codon stop_codon feature/stop feature/cds CDS |