BBa_K1915002

BBa_K1915002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1915002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tatenda Tela

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 11:08:34

LTNF15

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1915002_sequence (Version 1)

Description

LTNF15 (Lethal Toxin Neutralizing Factor) is a 10 residue peptide that has been shown to neutralize toxins from snakes, scorpions, and bacteria. LTNF peptides were first isolated from opossum (Didephis virginiana) serum. This part contains the LTNF15 peptide as well as C-term Myc and 6xHis tags that can be used for purification and then later removed using the HRV (Human Rhinovirus) protease.

Notes

N/A

Source

The sequence for the LTNF peptides were obtained from (G Chavan, et. al. (2014). Protein and peptide letters)
The full sequence was synthesized by IDT.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LTNF15	1,45	CDS, feature/protein
HRV 3C site	46,69	feature/binding, non_covalent_binding_site
Myc tag	70,99	feature/tag, tag
6 His	115,132	feature/tag, tag
stop codon	133,135	stop_codon, feature/stop

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1915002/1

Member of these Collections

• iGEM Parts Registry