Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zekun Lv
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-18 10:30:22
A temperature promoter cotaining clts repressor
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1919101_sequence (Version 1) |
Description
This is based on part(BBa_K608351).Composite promoter (BBa_K608351) include the mutant type CIλrepressor so that it can express above 37℃.Compared with the part(BBa_K608351),it is more sensitive at 37℃.According to the essay, we choose five important sites A44G, G92A, C188T, G378A, A629T, which may enhance more than tenth times expression at 37℃，compared with wild type(BBa_K608351).Notes
we choose five important sites A44G, G92A, C188T, G378A, A629T to mutant.Source
It is synthtetised by Tsingke.| Sequence Annotation | Location | Component / Role(s) |
| mutant-cIts | 62,775 | feature/cds CDS |