Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhuozhi Chen
Date created: 2016-09-30 11:00:00
Date modified: 2016-10-19 11:21:56
GCW51
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1921006_sequence (Version 1) |
Description
As a cell wall protein of Pichia pastoris, GCW51 is often used as an anchor protein in Pichia pastoris surface display system because of its stable connection with the dextranomer in cell wall.?? By fusing GCW51 with the target protein expression, the target protein can be displayed on the outer of the yeast cell wall.??By expressing the fusion protein of GCW51 protein and PETase, PETase was expressed on the surface of Pichia pastoris, and the whole cell catalyst for the degradation of PET was obtained. We made the PETase fixed on the cell wall, which can improve the stability of PETase, and is easy to control the degradation reaction of PET and PETase recycling.