BBa_K1923011

BBa_K1923011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1923011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianyang Mao
Date created: 2016-10-23 11:00:00
Date modified: 2016-10-24 10:06:13

Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1923011_sequence (Version 1)

Description

This composite part is a fusion protein composed of GAL4, triple FLAG tag, two SV40 nuclear localization sequence,dCas9 and GFP. Triple FLAG tag and GFP is designed for observation and expression detection. The protein produced by this parts can be sequestered in the cytoplasm with the help of sgRNA and PAMmer, utilizing dCas9's mRNA binding ability. However, when there is a mutation in the target sequence of the mRNA, sv40 NLS would drag this protein into the nuclear. Then GAL4 would active the gene downstream UAS promoter. Thus this parts can be used for gene mutation surveillance [1].

Notes

This part is listed for the gold medal criterion (Improve the function OR characterization of a previously existing BioBrick Part or Device).

Source

This part was assembled from previous BioBricks (BBa_K1323002, BBa_K1493504 and BBa_K1470002) originating from distribution kits, and was integrated into a fusion protein.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1923011/1