BBa_K1926001

BBa_K1926001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1926001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyi Liu

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-19 01:15:47

A cyclic promoter of Cyclin E from human genome

Details

• Types: DnaRegion
• Roles: engineered_region; Project
• Sequences: BBa_K1926001_sequence (Version 1)

Description

This part is a cyclic promoter named Cyclin E from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[1,2]

1. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.
2. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.

Notes

None

Source

The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:
pCCNE-F: CGTGTTTACATTCCACCCGCGCCA
pCCNE-R: TGATGGGGCTGCTCCGGCCT
(Product: 986)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P CCNE	65,1049	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1926001/1

Member of these Collections

• //chassis/eukaryote/human
• //collections
• //promoter
• iGEM Parts Registry