Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinyi Liu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-11 07:02:32
The SNAP-mCHERRY- CYAN UNIT
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1926031_sequence (Version 1) |
Description
This part is the SNAP-mCHERRY-CYAN UNIT of cyclebow including a SNAP UNIT, a mCHERRY UNIT and a plain CYAN. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox.You may use this part to:
1) Test the cut-off efficiency of recombinase CRE and VIKA;
2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair;
3) Fast cut off this part by transient transfecting recombinase gene into nucleus;
4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
Notes
NoneSource
The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira. CYAN was got from commercial plasmid: puc18 cfp-yfp.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1926022 CYAN mutate C into A to remove Xba1 | 1,1704 1712,2428 1707,1707 | feature/BioBrick engineered_region CDS feature/cds feature/mutation sequence_alteration |