CiproR

BBa_K1930004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1930004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mareike Schmidt
Date created: 2016-10-15 11:00:00
Date modified: 2016-10-16 04:58:30

Ciprofloxacin resistance cassette



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1930004_sequence (Version 1)

Description

QnrS1 resistance cassette BioBrick BBa_K1930004.

Notes

To design a QnrS1 resistance cassette BioBrick BBa_K1930004 we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original QnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+QnrS1+2TER.

Source

Ordered as gBlock. IDT (Integrated DNA technologies).

Sequence Annotation Location Component / Role(s)
P(AtpI)
BBa_K1930005
Qnrs1
2TER
BBa_B0015
1,350
1,350
351,1007
1008,1136
1008,1136
feature/promoter promoter
feature/BioBrick engineered_region
CDS feature/cds
stop_codon feature/stop
engineered_region feature/BioBrick
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1930004/1