Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eike Mahlandt
Date created: 2016-10-15 11:00:00
Date modified: 2016-10-16 04:58:48
PAtpI
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1930005_sequence (Version 1) |
Description
The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinaiet al.]. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.
Notes
The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinaiet al.]. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.