Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Barbora Waclawikova
Date created: 2016-10-15 11:00:00
Date modified: 2016-10-17 09:01:57
Ciprofloxacin resistance cassette in integration plasmid (BBa_K823023)
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1930007_sequence (Version 1) |
Description
This part is a ciprofloxacin resistance cassette. It is containing a PAtpI constitutive promoter, ciprofloxacin resistance gene qnrS1 and double terminator.
The promoter PAtpI (BBa_K1930005) is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.
Ciprofloxacin resistance gene qnrS1 is found naturally in E. coli, and other Gram-negative strains. qnr genes code for pentapeptide repeat proteins.??These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones.
Double terminator (BBa_B0015) is the most commonly used terminator. For more information see here: http://parts.igem.org/Part:BBa_B0015.
Notes
designSource
Ciprofloxacin resistance cassette: Ordered as gBlock. IDT (Integrated DNA technologies).
BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023.
| Sequence Annotation | Location | Component / Role(s) |
| P(AtpI) BBa_K1930005 QnrS1 2TER BBa_K823023 cat amyE bla amyE BBa_B0015 | 1,350 1,350 351,1007 1008,1136 1137,7198 1473,2123 2494,3522 5476,6336 6661,7181 1008,1136 | feature/promoter promoter feature/BioBrick engineered_region CDS feature/cds stop_codon feature/stop engineered_region feature/BioBrick feature/cds CDS sequence_feature feature/misc feature/cds CDS feature/misc sequence_feature feature/BioBrick engineered_region |