scFv

BBa_K1933002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1933002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tomoki Uchino

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-18 10:28:59

anti-Norovirus GII.4 scFv

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1933002_sequence (Version 1)

Description

This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens. [1] This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project.
For more information, please visit our Wiki page.

Notes

silent mutation was made to remove PstI: G255C

Source

We have been given given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start codon	1,3	feature/start, start_codon
VH	4,392	CDS, feature/cds
linker	393,438	feature/protein, CDS
VL	439,777	feature/cds, CDS
stop codon	778,780	stop_codon, feature/stop
G255C	255,255	point_mutation, feature/s_mutation

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1933002/1

Member of these Collections

• //function/immunology
• iGEM Parts Registry