Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-17 12:24:17
Epsilon/MazF : toxin and antitoxin
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1937009_sequence (Version 1) |
Description
(Chassis E. coli, carrier plasmid pSB1C3)Length: 1047 bp
Background:
This Epsilon/MazF part is one of the two components of a double toxin-antitoxin system. The part was designed to be carried on a plasmid while its counterpart MazE/Zeta was carried on another. Unfortunately, only Epsilon/MazF part was successfully cloned in E. coli.
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
Notes
Epsilon and MazF are under the control of the promoter Pveg (constitutive promotor for B. subtilis) followed by a strong RBS. A theophylline-dependent riboswitch was used to prevent the MazE toxin expression during the cloning steps in presence of theophylline (Topp and Gallivan, 2008). Indeed, with an expressed toxin, it would be impossible to clone the Biobrick in E. coli.SacII and SalI are the restriction sites placed respectively before and after the riboswitch so that this fragment can easily be replaced depending on the control we want to apply to the bacteria.Source
The gene Epsilon codes for a toxin countered by the anti-toxin Zeta (Zielenkiewicz and Cegłowski, 2005 ; Mutschler et al., 2011). The gene MazF encodes the anti-toxin for the MazE toxin (Bravo et al., 1987, Zhang et al., 2005, Wang et al., 2013).| Sequence Annotation | Location | Component / Role(s) |
| Pveg Antitoxin Epsilon RBS Antitoxin RBS toxin Riboswitch Terminator Toxin mazF | 1,237 250,522 238,249 570,579 529,579 925,1004 586,924 | feature/promoter promoter feature/protein CDS ribosome_entry_site feature/rbs ribosome_entry_site feature/rbs feature/misc sequence_feature feature/stop stop_codon CDS feature/protein |