BBa_K194000

BBa_K194000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K194000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Christian Kaas

Date created: 2009-07-12 11:00:00

Date modified: 2015-05-08 01:11:16

cln2 PEST destabilization domain for rapid protein turnover

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K194000_sequence (Version 1)

Description

C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes.

Notes

The construct follow the Silverfusion standard

Source

Saccharomyces cerevisiae genomic DNA, Genbank Accession M33265

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Cln2 Coding sequence	1,537	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K194000/1

Member of these Collections

• iGEM Parts Registry