BBa_K194002

BBa_K194002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K194002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Christian Kaas

Date created: 2009-07-12 11:00:00

Date modified: 2015-05-08 01:11:16

Destabilized GFP for yeast

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K194002_sequence (Version 1)

Description

This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000.
In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.

Notes

follows standard biobrick standards

Source

Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry"

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
yeast GFP CDS	1,707	CDS, feature/cds
cln2 PEST domain CDS	708,1257	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K194002/1

Member of these Collections

• iGEM Parts Registry