Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Claire raynaud, Yoann Santin, Louis Givelet
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-11 05:44:37
desA, lysine decarboxylase coding sequence
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K1951000_sequence (Version 1) |
Description
This DNA sequence codes Lysine descarboxylase (Streptomyces coelicolor) is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid.There is cadeverin producing (1,5-diaminopentane), a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.
In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.
This enzyme is also the first step in the production of desferrioxame B which is a siderophore.
Notes
The DNA sequence was modified by the addition of a prefix and suffix sequences (containing the restriction site EcoRI, XbaI and SpeI, PstI respectively) and was inserted in a promoteur using SLIC method.Source
This part comes from the genome sequencing of Streptomyces coelicolor. The following website enabled to find the gene reference and its chromosomal position. http://strepdb.streptomyces.org.uk/cgi-bin/dc3.pl?accession=AL645882&serial=2769&width=900&start=3031362&end=3041362&iorm=mapThe second website below is the source of the DNA sequence we have used.
http://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3
| Sequence Annotation | Location | Component / Role(s) |
| desA double stop | 1,1440 1440,1446 | engineered_region feature/BioBrick stop_codon feature/stop |