BBa_K1954006

BBa_K1954006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1954006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kamil Żmijewski

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-25 07:13:55

Feedback-resistant N-acetylglutamate synthetase

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1954006_sequence (Version 1)

Description

N-acetylglutamate synthetase (NAGS) catalyzes the first step of the linear biosynthetic pathway of L-arginine as follows:

acetyl-coA + L-glutamate = coA + N-acetyl-L-glutamate

This sequence is the open reading frame of the feedback resistant mutant PT2M217 NAGS which is not responsive to the inhibition by L-arginine present in the endogenous system.

Notes

One restriction site was removed from the source sequence by silent mutagenesis as follows:

PstI 421: Leu 121 (CTG) 46.9 % -> Leu (TTA) 15.2 %

Source

AF008119.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I14033	1,38	promoter, feature/promoter
BBa_B0034	39,50	engineered_region, feature/BioBrick
frNAGS	57,1388	feature/cds, CDS
BBa_B0014	1400,1494	feature/stop, stop_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1954006/1

Member of these Collections

• iGEM Parts Registry