BBa_K1957004

BBa_K1957004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1957004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nancy Teng
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-06 10:19:05

HydA subunit of FeFe Hydrogenase



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1957004_sequence (Version 1)

Description

One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydA subunit which is the membrane bound catalytic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.

Notes

This is one of of the three FeFe Hydrogenase subunits. To make the entire FeFe Hydrogenase construct the following BioBricks need to be ligated with BBa_K1957004: BBa_K1957001 and BBa_K1957002 or BBa_K1957001 and BBa_K1957003. pSB1C3 would not conjugate properly into S.oneidensis, thus the three subunits were ligated using golden gate cloning into the pBAD expression vector.

Source

The source of the part will be its sequence which was retrieved from GenBank.

igem#status
Planning
 
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user/james
 
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user/myers
 
synbiohub#topLevel
BBa_K1957004/1