BBa_K1959003

BBa_K1959003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1959003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Junyu Chen

Date created: 2016-10-03 11:00:00

Date modified: 2016-10-24 02:02:32

BKT coding sequence fused with Pea transit peptide

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1959003_sequence (Version 1)

Description

This part is consist of a Pea transit peptide and the coding sequence of beta-carotene ketolase originally derived from
Chlamydomonas reinhardtii. Beta-carotene ketolase realizes the convertion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of beta-carotene ketolase has been optimized for better expression in Orazy sativa.

Notes

A pea transit peptide has been functionally fused to the BKT cds. The BKT codon has made some optimization for expression in rice. An Pst I restriction site has been mutated by site-directed mutation.

Source

Original sequence can be accessed from Genebank, Genebank accession: AY860820.1.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Pst I mutant	1036,1041	feature/s_mutation, point_mutation
BKT	172,1158	feature/cds, CDS
Pea transit peptide	1,171	feature/cds, CDS
Initiation codon of BKT	172,174	feature/start, start_codon
Initiation codon of Pea transit peptide	1,3	start_codon, feature/start
Termination codon of BKT	1156,1158	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1959003/1

Member of these Collections

• iGEM Parts Registry