BBa_K1963011

BBa_K1963011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1963011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Fatima Ulhuq

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-18 04:16:20

An sRNA system for down-regulation of Serratia marcescens fliC encoding flagellin.

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K1963011_sequence (Version 1)

Description

This is a multi-sequence system for small RNA (sRNA) production in an Serratia marcescens host - or especially with co-expression of Serratia marcescens Hfq BBa_K1963001. The system is based on the BBa_K1963003 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the S. marcescens chiA hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of S. marcescens.

Notes

N/A

Source

This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
proD promoter	1,193	promoter, feature/promoter
fliC targeting sRNA	194,229	non_covalent_binding_site, feature/binding
chiA loop	230,271	stem_loop, feature/stem_loop
terminator	272,389	sequence_feature, feature/misc

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1963011/1

Member of these Collections

• iGEM Parts Registry