BBa_K1964002

BBa_K1964002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1964002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Gerlinger

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 12:48:22

FliC signal sequence

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_K1964002_sequence (Version 1)

Description

FliC encodes for Flagellin, the main compound of the E. coli flagellum. The 178bp FliC signal sequence directs it to the cell membrane, where it usually gets integrated.
In order to utilize this highly regulated mechanism, we generated a FliCD-knockout E. coli strain. This enabled us to fuse the signal sequence to the N-terminal end of our protein of interest, which as a result got exported and secreted into the medium.

Notes

Requires the design of an E. coli strain where FliC and FliD are both knocked out.

Source

E. coli MG1655 gDNA

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1964002/1

Member of these Collections

• iGEM Parts Registry