Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ibrahim Al-Masoud
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-19 03:05:34
ErmE promoter Phytobrick
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1968008_sequence (Version 1) |
Description
A widely used constitutive promoter, ErmE (promoter region of erythromycin-resistance gene) was isolated from Streptomycin (Bibb et al., 1985). It works with related gram positive actinobacteria, such as Rhodococcus. We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM???s pairwise assembly standard.References:
Bibb, M.J., Janssen, G.R. and Ward, J.M., 1985. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene, 38, pp.215-226.
Tong, Y., Charusanti, P., Zhang, L., Weber, T. and Lee, S.Y., 2015. CRISPR-Cas9 based engineering of actinomycetal genomes. ACS synthetic biology, 4, pp.1020-1029.
Notes
The part was integrated in reverse and we advise the user to use the reverse complement sequence for downstream applications.Source
This part was extracted through PCR from pCRISPR-Cas9 vector (GenBank accession number KR011749) (Tong et al., 2015) and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500 or pUPD2). The sequence of this part was confirmed by Sanger Sequencing.| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick Adapter Site A ErmE Phytobrick Adapter Site B | 1,4 5,70 71,74 | feature/misc sequence_feature promoter feature/promoter feature/misc sequence_feature |