Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandra Adriana Schiavon Osorio
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-17 10:56:53
PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1968013_sequence (Version 1) |
Description
Glyceraldehyde-3-phosphate dehydrogenase (GPDA) is an enzyme that catalyzes the sixth step of glycolysis. The gene gpdA is constitutively expressed in A. nidulans (Punt et al., 1990). Moreover, the gpdA promoter is able to drive robust transcription of heterologous genes. Intracellularly expressed model heterologous proteins like the E. coli b-galactosidase (lacZ) and b-glucuronidase (uiaA) under the control of the gpdA promoter can account for up to 10???25% of soluble protein in A. niger (Punt, Zegers, Busscher, Pouwels, & van den Hondel, 1991). The ability to drive constitutive tran- scription makes the gpdA promoter particularly useful for expression, under varying cultures conditions, of selection markers and reporter genes, many of which are from heterologous sources.Notes
Phytrobrick format and MoClo compatible (Overhangs E and C)Source
Genomic Sequence. The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection.| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick Adapter Site E Phytobrick Adapter Site C PgdaA | 1,4 678,681 5,677 | feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter |