Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Harrigan
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:18
{VtaA11 AtD}
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K197004_sequence (Version 1) |
Description
This is an Autotransporter of the AT-2 family from the organism Haemophilus parasuis. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.Notes
N/ASource
Pool O09ig113 through O09ig120, assemble by PCA
PCR O09ig113/O09ig120 on PCA reaction (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig113 {?VtaA11 AtD?}
O09ig113 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG
O09ig114 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT
O09ig115 PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT
O09ig116 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA
O09ig117 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA
O09ig118 PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA
O09ig119 PCA assembly of {?VtaA11 AtD?} (B09ig113)
ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG
O09ig120 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC