Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Harrigan
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:18
{Hag AtD}
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K197005_sequence (Version 1) |
Description
This is a 200kDa protein with 10-stranded beta barrel. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.Notes
N/ASource
Pool O09ig121 through O09ig128, assemble by PCA
PCR O09ig121/O09ig128 on PCA reaction (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig121 {?Hag AtD?}
O09ig121 PCA assembly of {?Hag AtD?} (B09ig121)
CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT
O09ig122 PCA assembly of {?Hag AtD?} (B09ig121)
CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG
O09ig123 PCA assembly of {?Hag AtD?} (B09ig121)
TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG
O09ig124 PCA assembly of {?Hag AtD?} (B09ig121)
CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA
O09ig125 PCA assembly of {?Hag AtD?} (B09ig121)
CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC
O09ig126 PCA assembly of {?Hag AtD?} (B09ig121)
TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA
O09ig127 PCA assembly of {?Hag AtD?} (B09ig121)
AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG
O09ig128 PCA assembly of {?Hag AtD?} (B09ig121)
CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT