BBa_K1973010

BBa_K1973010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriel Ruiz Romero
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 09:35:33

miniTn7BB-Gm-nahR-Psal-nasF-yhjH



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1973010_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. yhjH (see BBa_K777121) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).

Notes

Designs are described in basic parts pages

Source

Basic parts were cloned into the miniTn7BB-Gm Backbone

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973010/1