Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriel Ruiz Romero
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 09:41:00
miniTn7BB-Gm-nahR-Psal-nasF
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1973019_sequence (Version 1) |
Description
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see Bba_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).Notes
Designs are described in basic parts pagesSource
Basic parts were cloned into the miniTn7BB-Gm Backbone| Access | Instance | Definition |
| public public | BBa_K510000 BBa_K1973005 | BBa_K510000 BBa_K1973005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510000 BBa_K1973005 | 1,4388 4397,5547 | BBa_K510000 BBa_K1973005 |