BBa_K1973026

BBa_K1973026 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973026
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriel Ruiz Romero
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 08:04:04

glpF (membrane transporter)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1973026_sequence (Version 1)

Description

Codifies the GlpF protein from Pseudomonas aeruginosa, which is a glycerol channel that increases the glycerol intake for bioremediation purposes.

Notes

It was amplified by mutagenic PCR. Nucleotide 693 (starting at ATG) was changed (from a cytosine to a thymine). We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts.

Source

Obtained by PCR from the genome of Pseudomonas aeruginosa PAO1

Sequence Annotation Location Component / Role(s)
B0034
Scar
glpF
C --> T
1,12
13,18
19,861
711,711
ribosome_entry_site feature/rbs
feature/scar
CDS feature/cds
sequence_alteration feature/mutation
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973026/1