Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YU-CHUN WU
Date created: 2016-10-12 11:00:00
Date modified: 2017-03-28 09:59:25
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1974021_sequence (Version 1) |
Description
By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), Hv1a, snowdrop lectin, and the 6xHistag (BBa_ K1223006). Moreover, we added two kinds of the linker to connect Hv1a, snowdrop-lectin. Two linkers are different size of amino acids. We can express the toxin fused with snowdrop lectin by IPTG induction.This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Omega-hexatoxin-Hv1a is coded for the venom of a spider, Hadronyche versuta.
It is under the control of the strong T7 promoter. Snowdrop lectin acts as a carrier that could transport the toxin to insect???s nervous system, hemolymph and can improve the oral activity. A 6xHistag is added for further protein purification.
Notes
It is under the control of the strong T7 promoter. Snowdrop lectin acts as a carrier that could transport the toxin to insect???s nervous system, hemolymph and can improve the oral activity. A 6xHistag is added for further protein purification.Source
Artificial Synthesis| Access | Instance | Definition |
| public public public | BBa_I712074 BBa_B0034 BBa_K1223006 | BBa_I712074 BBa_B0034 BBa_K1223006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_B0034 BBa_K1223006 | 1,46 55,66 75,101 | BBa_I712074 BBa_B0034 BBa_K1223006 |