Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YU-CHUN WU
Date created: 2016-10-12 11:00:00
Date modified: 2017-03-28 10:01:36
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1974023_sequence (Version 1) |
Description
By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), Hv1a, snowdrop lectin, and the 6xHistag (BBa_ K1223006). Moreover, we added two kinds of the linker to connect Hv1a, snowdrop-lectin. Two linkers are different size of amino acids. We can express the repellent peptide fused with snowdrop lectin by IPTG induction.Notes
It is under the control of the strong T7 promoter. Snowdrop lectin acts as a carrier that could transport the peptide to insect???s nervous system, hemolymph and can improve the oral activity. A 6xHistag is added for further protein purification.Source
Artificial Synthesis| Access | Instance | Definition |
| public public public | BBa_I712074 BBa_B0034 BBa_K1223006 | BBa_I712074 BBa_B0034 BBa_K1223006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_B0034 BBa_K1223006 | 1,46 55,66 75,101 | BBa_I712074 BBa_B0034 BBa_K1223006 |