BBa_K1981002

BBa_K1981002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1981002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yulei Dang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 06:22:54

lsrB



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1981002_sequence (Version 1)

Description

Autoinducer 2-binding protein LsrB. Membrane channel protein. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Binds AI-2 and delivers it to the LsrC and LsrD permeases (Probable). Share the same promoter with lsrA. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.

Notes

No point mutation was performed.

Source

Sequence was acquired from genomic of Bacillus thuringiensis serovar thuringiensis str. IS5056. Part was acquired by OE-PCR.

Sequence Annotation Location Component / Role(s)
ATG Start Codon
lsrB Coding Region
TAG Stop Codon
1,3
1,1044
1042,1044
start_codon feature/start
feature/cds CDS
feature/stop stop_codon
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1981002/1