BBa_K1982009

BBa_K1982009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1982009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zexu Li
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 12:15:06

Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1982009_sequence (Version 1)

Description

Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS.
Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].

Notes

mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg

Source

The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.

Sequence Annotation Location Component / Role(s)
atg
Eukaryotic rbs
19,21
1,18
start_codon feature/start
ribosome_entry_site feature/rbs
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1982009/1