BBa_K1983002

BBa_K1983002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1983002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Vykintas Jauni??kis

Date created: 2016-09-13 11:00:00

Date modified: 2016-10-16 04:36:39

gp45 phenylalanine mutant M6 with C-terminal 6XHis-Tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1983002_sequence (Version 1)

Description

T4 phage clamp protein gp45 with 6 stable mutations to phenylalanine.

Notes

Designed including NdeI restriction site for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.

Source

T4 phage

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
gp45M6	4,687	CDS, feature/protein
C-terminal 6XHis-Tag	688,711	tag, feature/tag
Double translation stop codon	712,714	feature/stop, stop_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1983002/1

Member of these Collections

• iGEM Parts Registry