Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jasmine Cornish
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 02:27:29
mamX his-tagged, signal sequence cleaved
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1985005_sequence (Version 1) |
Description
Usage: The mam gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the protein is exposed to into in solution. This sequence produces a soluble protein because it has the membrane anchor cleaved and is targeted to the secretory pathway, which will leave the protein in the periplasm. It was expressed, purified and then exposed to iron.Biology:
Mam is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
Notes
Codon OptimisedSource
The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.| Sequence Annotation | Location | Component / Role(s) |
| SecS-sol-mamX | 15,794 | feature/cds CDS |