BBa_K199034

BBa_K199034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K199034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alyndria Thompson
Date created: 2009-07-12 11:00:00
Date modified: 2015-05-08 01:11:19

RFP with CGGUC Addition



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K199034_sequence (Version 1)

Description

This RFP reporter gene has a 5-base pair CGGUC addition after the start codon and before the rest of the gene. This is used in conjunction with the CGGUC tRNA (BioBrick part K199028). This CCACU tRNA suppresses the frameshift caused by this 5-base pair addition in the reporter.

Notes

We positioned the ATG before the 5-base codon and continued with the rest of the RFP gene. This way, the RBS wouldn't read the reporter gene without reading over the 5-base codon first. In order to ligate our 5-base pair addition at the desired position in the reporter gene, we utilized the restriction enzyme NcoI which cut at a single site 424 bp into the gene

Source

The template of the gene comes from BioBrick part E1010. We designed a forward primer that included the first 20 nucleotides on the 5' end, and a reverse primer that included the last 20 nucleotides on the 3' end of the RFP gene.

Sequence Annotation Location Component / Role(s)
5 base pair addition
Nco1 restriction site
25 bp addition
Double Stop
4,9
424,429
687,711
681,686
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K199034/1