Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chen, Pei-En
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 03:21:00
Pcons-RBS-LO-AOX2-His
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1991009_sequence (Version 1) |
Description
A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip.Notes
NoSource
E. coli (Lpp-OmpA) & Pichia pastoris (AOX)| Access | Instance | Definition |
| public public public | BBa_J23101 BBa_B0034 BBa_K1991006 | BBa_J23101 BBa_B0034 BBa_K1991006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_B0034 BBa_K1991006 | 1,35 44,55 62,2662 | BBa_J23101 BBa_B0034 BBa_K1991006 |