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BBa_K1997005

BBa_K1997005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1997005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyuan Qiu

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-16 10:16:35

sHRP-N->FRB->RBS->FKBP-> sHRP-C

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_K1997005_sequence (Version 1)

Description

This is the C-terminal of split Luciferase reporter. It can be used for protein-protein interaction researches.

Notes

This part is codon optimized for expression in E.coli This part is codon optimized for expression in E.coli, a 10aa linker was added to the both sides of the cds

Source

This part is custom synthesized, the original sequence was acquired form firefly

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
sHRP-N 10aa linker FRB RBS FKBP 10aa linker sHRP-C	1,641 642,671 684,968 969,998 999,1322 1335,1364 1365,1653	feature/cds CDS CDS feature/protein feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS CDS feature/protein feature/cds CDS