BBa_K1999000

BBa_K1999000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1999000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jimin Sun
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 03:54:41

Poly(ethylene terephthalate) hydrolase (PETase)



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1999000_sequence (Version 1)

    Description

    Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Catalyzes the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Is also able to hydrolyze bis(hydroxyethyl) terephthalate (BHET) to yield MHET with no further decomposition. Shows low activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) in vitro.

    Notes

    N/A

    Source

    Ideonella sakaiensis (strain 201-F6)

    Sequence Annotation Location Component / Role(s)
    ATG
    PETase
    TAA
    		1,3
    1,870
    871,873
    		start_codon feature/start
    CDS feature/cds
    stop_codon feature/stop

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1999000/1
     

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